ABSTRACT
Objective To explore the effect of ginsenoside Rg1 on proliferation of neural stem cells (NSCs) in vitro. Methods The NSCs cultures were generated from the brain of embryonic day 16 SD rat. Primitive NSCs were cultured, proliferated and passaged. The NSCs were identified by the immunocytochemical (ICC) staining of Nestin. The ICC staining of BrdU was adopted to characterize the proliferation of NSCs. According to limited dilution method, the effect of ginsenoside Rg1 on the proliferation of NSCs was observed. Results The NSCs were successfully cultured and proliferated in vitro. Different dosages (100, 4, 8 ?mol/L) of ginsenoside Rg1 could promote the proliferation of NSCs in vitro. Compared with the control group, the numbers of nerurospheres of the three dosage ginsenoside Rg1 groups (40, 4, 0.4 ?mol/L) were increased obviously. Conclusions Ginsenoside Rg1 significantly promote the proliferation of NSCs in vitro.
ABSTRACT
Aim To explore the effects of astragaloside(AS)on the proliferation of neural stem cells(NSCs).Methods The NSCs of embryonic day(E)16 SD rat were cultured in vitro.The NSCs were identified by the immunocytochemical(ICC)staining of Nestin.The ICC staining of BrdU was adopted to characterize the proliferation of NSCs.The number of neurospheres and the ICC staining of BrdU were performed to identify their proliferation properties.Real time RT-PCR technique was used to investigate the proliferation mechanisms of the AS on the NSCs.Results Characteristic protein(Nestin)of NSCs labeling,BrdU labeling were positive showed by immunocytochemical staining.The ICC of BrdU labeling results showed that different dosages of AS could promote the proliferation of NSCs in vitro.The proliferation rate of NSCs was increased extremely significant(P